However, immune cell heterogeneity at the single-cell level, and how immune cell states shape transplantation immunity, remain incompletely characterized. Immune cells play important roles in mediating allograft rejection and tolerance after cardiac transplantation. Credit: Cardiovascular Innovations and Applications (2023). t-SNE maps indicating the expression of selected well-established cellular markers in cell populations identified as (F), T cells, (G), B cells, (H), DC cells, and (I), NK cells. Top ten upregulated DEGs (ranked by log fold change) of each cluster in immune cells, plotted in a heatmap. Heatmap analysis with known gene expression profiles of T cells, B cells, DC cells, NK cells, and nuocytes. Cluster map showing the assigned identity for each cluster defined in (B). t-SNE projection of 7967 single immune cells (each point represents a single cell) in lymph nodes from syngeneic cardiac transplanted mice sharing similar transcriptome profiles, grouped by color according to unsupervised clustering results. Single-cell RNA sequencing was applied to immune cells of lymph nodes derived from cardiac transplanted mice, and the output data were used for expression analyses. Single-Cell RNA Sequencing of Immune Cells in Lymph Nodes Reveals the Presence of 11 Cell Clusters.
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